Cromatest APTT Reagent

The capacity of blood to form a fibrin clot by means of the intrinsic hemostatic pathway requires coagulation factors I, II, V, VIII, IX,  X, XI and XII, platelet lipids and calcium4. The assay is performed by the addition in the sample of a suspension of rabbit brain cephalin with a surface activator1.

The APTT has proven to be a simple and highly reliable measurement of the intrinsic coagulation mechanism5.

Reagent Composition

APTT

Rabbit brain cephalin and ellagic acid activator with buffer. Stabilizers and preservatives.

Optative.

  • Calcium Chloride 0.02 mol/L Ref 3510401
  • Plasma Control Level 1 Ref. 3520101,
  • Plasma Control Level 2 Ref. 3520201.

Storage and Stability

Store at 2-8ºC. The reagent is stable until the expiry date stated on the label.

The reconstituted APTT reagent is stable for 5 days when stored in the original container at 2-8ºC. Do not freeze.

Reagent Preparation

  1. Reconstitute the contents of APTT with 4,0 mL of distilled water.
  2. Replace the stopper and thoroughly mix the vial contents. Let stand for no less than 30 minutes prior to use to assure complete hydration of the contents.

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Reagent Preparation

  1. Reconstitute the contents of APTT with 4,0 mL of distilled water.
  2. Replace the stopper and thoroughly mix the vial contents. Let stand for no less than 30 minutes prior to use to assure complete hydration of the contents.